The sample, or a dilution of it, is filtered through a 0.45 μm membrane filter, after which the membrane filter is anaerobically incubated at 44 °C on a medium with such selective characteristics thatvegetative cells and traces of Clostridium perfringens will form characteristic colonies. Confirmation of the typical colonies is executed by MALDI-TOF. |
Matrices qualified by the Dutch RvA: | Drinking water |
Reporting limit: | 1 kve/100ml, matrix dependent |
Measurement uncertainty: | 35% |
Sustainability: | 1 day
, if conserved with NTA and Na-thiosulphate
|
Sampling bottle: | Plastic
, white
, 500 ml
, conservation with NTA and Na-thiosulphate
(fill up to the neck of the bottle)
|