WLN-BM.W.40.1Determination of the number of Clostridium perfringens; membrane filtrationConform NEN-ISO 14189
Principle:The sample, or a dilution of it, is filtered through a 0.45 μm membrane filter, after which the membrane filter is anaerobically incubated at 44 °C on a medium with such selective characteristics thatvegetative cells and traces of Clostridium perfringens will form characteristic colonies. Confirmation of the typical colonies is executed by MALDI-TOF.
Determination:Clostridium perfringens
Performance characteristics:
Matrices qualified by the Dutch RvA:Drinking water
Reporting limit:1 kve/100ml, matrix dependent
Measurement uncertainty:35%
Sustainability:1 day , if conserved with NTA and Na-thiosulphate
Sampling bottle:Plastic , white , 500 ml , conservation with NTA and Na-thiosulphate (fill up to the neck of the bottle)
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[ 10-11-2023 10:58:37 ]