WLN-BM.W.01.2	Determination of bacteria of the coli group in water by means of membrane filtration (standard test).	equivalent to NEN-EN-ISO 9308-1
Principle:	The sample is filtered through a suitable membrane filter. Next the filter, after a short incubation at 25 °C, is incubated at 37 °C on a solid, lactose-containing medium (LSA) with such selectivecharacteristics that bacteria of the coli group will form characteristic colonies. The suspected characteristic colonies are confirmed by means of MALDI-TOF.Equality compaired to the NEN relates to the use of LSA medium instead of CCA. The confirmation with classic methods is replaced by confirmation using the MALDI-TOF.
Determination:	Coliform bacteria at 37C
Performance characteristics:
Matrices qualified by the Dutch RvA:	Drinking water, Groundwater
Reporting limit:	1 kve/100ml, matrix dependent
Measurement uncertainty:	35%
Sustainability:	1 day , if conserved with NTA and Na-thiosulphate
Sampling bottle:	Plastic , white , 500 ml , conservation with NTA and Na-thiosulphate (fill up to the neck of the bottle)

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