A diluted sample is pipeted in a series of microtiter plate cups, containing dehydrated medium that contains 4-methylumbelliferyl-ß-D-glucoside (MUD). The microtiter plate is examined in the dark under aUV lamp at a wavelength of 365 nm, after an incubation of 36 hours at 44 °C. The presence of Enterococcus is indicated by a blue fluorescence as result of the hydrolysis of MUD. Theresults are expressed in MPN (Most Probable Number) per 100 ml. |
Matrices qualified by the Dutch RvA: | Surface water |
Reporting limit: | 15 kve/100ml, matrix dependent |
Measurement uncertainty: | 60% |
Sustainability: | 1 day
, if conserved with NTA and Na-thiosulphate
|
Sampling bottle: | Plastic
, white
, 500 ml
, conservation with NTA and Na-thiosulphate
(fill up to the neck of the bottle)
|