| A typical colony is suspended in a cup containing the required reagents. A fragment of any present DNA is multiplied many times in the PCR. The amplified substance obtained from this fragment is detected and identified by means of a light emitting part (probe). For this confirmation method the 100%-PCR inspection is used. With this inspection we can guarantee the PCR reaction for each confirmation separately. If the 100%-PCR inspection gives a negative result, this will indicate that no PCR reaction was possible or that no colony has beenadded to the mixture. |
