The sample is filtered through a suitable membrane filter. Next the filter is treated ultrasonic. For elimination of disturbing flora one part of the concentrate is pasturised at 50°C and one part istreated with acid. The non pasturised, pasturised and acid treated concentrate is placed and spreaded on different solid, selective mediawith selective characteristics and incubated at 37 °C for 7-10days.The Legionella bacteria will form characteristic colonies. The suspectcolonies are confirmed by MALDI-TOF. |
Matrices qualified by the Dutch RvA: | Cooling water, Surface water |
Reporting limit: | 100 kve/l, matrix dependent |
Measurement uncertainty: | 60% |
Sustainability: | 2 days
, if conserved with NTA and Na-thiosulphate
|
Sampling bottle: | Plastic
, white
, 375 ml
, conservation with NTA and Na-thiosulphate
(half warm and half cold water)
|