|
| Urea is converted, in the presence of the enzyme urease, into ammonia and carbon dioxide. The formed and already present ammonia will convert a-ketoglutarate, in the presence of a surplus of glutamate dehydrogenase (GLDH) and reduced nicotinamide adenine dinucleotide (NADH), into L-glutamate. In this reaction NADH is consumed. The quantity of NADH consumed in the reaction corresponds with the quantity of ammonia and half of the original quantity of urea. The remaining quantity of NADH is measured photometrically at 340 nm. |
|
|
| Matrices qualified by the Dutch RvA: | Swimmingpool water |
| Reporting limit: | 0.3 mg/l, matrix dependent |
| Measurement uncertainty: | 20% |
| Sustainability: | 12 days
|
| Sampling bottle: | Plastic
, dispotube
, 10 ml
(fill up to the neck of the bottle)
|
